GSTDTAP

浏览/检索结果: 共44条,第1-10条 帮助

已选(0)清除 条数/页:   排序方式:
澳大利亚AIMS发布《2023-2024年度珊瑚礁状况年度总结报告》 快报文章
资源环境快报,2024年第16期
作者:  魏艳红
Microsoft Word(24Kb)  |  收藏  |  浏览/下载:688/3  |  提交时间:2024/09/01
AIMS  Coral Reef Condition  GBR  Coral Bleaching  
Coupling delay controls synchronized oscillation in the segmentation clock 期刊论文
NATURE, 2020
作者:  Yoshioka-Kobayashi, Kumiko;  Matsumiya, Marina;  Niino, Yusuke;  Isomura, Akihiro;  Kori, Hiroshi;  Miyawaki, Atsushi;  Kageyama, Ryoichiro
收藏  |  浏览/下载:16/0  |  提交时间:2020/07/03

Individual cellular activities fluctuate but are constantly coordinated at the population level via cell-cell coupling. A notable example is the somite segmentation clock, in which the expression of clock genes (such as Hes7) oscillates in synchrony between the cells that comprise the presomitic mesoderm (PSM)(1,2). This synchronization depends on the Notch signalling pathway  inhibiting this pathway desynchronizes oscillations, leading to somite fusion(3-7). However, how Notch signalling regulates the synchronicity of HES7 oscillations is unknown. Here we establish a live-imaging system using a new fluorescent reporter (Achilles), which we fuse with HES7 to monitor synchronous oscillations in HES7 expression in the mouse PSM at a single-cell resolution. Wild-type cells can rapidly correct for phase fluctuations in HES7 oscillations, whereas the absence of the Notch modulator gene lunatic fringe (Lfng) leads to a loss of synchrony between PSM cells. Furthermore, HES7 oscillations are severely dampened in individual cells of Lfng-null PSM. However, when Lfng-null PSM cells were completely dissociated, the amplitude and periodicity of HES7 oscillations were almost normal, which suggests that LFNG is involved mostly in cell-cell coupling. Mixed cultures of control and Lfng-null PSM cells, and an optogenetic Notch signalling reporter assay, revealed that LFNG delays the signal-sending process of intercellular Notch signalling transmission. These results-together with mathematical modelling-raised the possibility that Lfng-null PSM cells shorten the coupling delay, thereby approaching a condition known as the oscillation or amplitude death of coupled oscillators(8). Indeed, a small compound that lengthens the coupling delay partially rescues the amplitude and synchrony of HES7 oscillations in Lfng-null PSM cells. Our study reveals a delay control mechanism of the oscillatory networks involved in somite segmentation, and indicates that intercellular coupling with the correct delay is essential for synchronized oscillation.


Monitoring cells of the mouse presomitic mesoderm using the Achilles reporter fused to HES7 sheds light on the mechanisms that underpin synchronous oscillations in the expression of clock genes between neighbouring cells.


  
Temporal Characteristics and Potential Sources of Black Carbon in Megacity Shanghai, China 期刊论文
JOURNAL OF GEOPHYSICAL RESEARCH-ATMOSPHERES, 2020, 125 (9)
作者:  Wei, C.;  Wang, M. H.;  Fu, Q. Y.;  Dai, C.;  Huang, R.;  Bao, Q.
收藏  |  浏览/下载:18/0  |  提交时间:2020/07/02
black carbon  temporal variation  source apportionment  meteorological condition  back trajectory  
Reconciling global priorities for conserving biodiversity habitat 期刊论文
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2020, 117 (18) : 9906-9911
作者:  Mokany, Karel;  Ferrier, Simon;  Harwood, Thomas D.;  Ware, Chris;  Di Marco, Moreno;  Grantham, Hedley S.;  Venter, Oscar;  Hoskins, Andrew J.;  Watson, James E. M.
收藏  |  浏览/下载:24/0  |  提交时间:2020/05/13
community  condition  conservation  contextual intactness  ecosystem  
Mutations that prevent caspase cleavage of RIPK1 cause autoinflammatory disease 期刊论文
NATURE, 2020, 577 (7788) : 103-+
作者:  Lalaoui, Najoua;  Boyden, Steven E.;  Oda, Hirotsugu;  Wood, Geryl M.;  Stone, Deborah L.;  Chau, Diep;  Liu, Lin;  Stoffels, Monique;  Kratina, Tobias;  Lawlor, Kate E.;  Zaal, Kristien J. M.;  Hoffmann, Patrycja M.;  Etemadi, Nima;  Shield-Artin, Kristy;  Biben, Christine;  Tsai, Wanxia Li;  Blake, Mary D.;  Kuehn, Hye Sun;  Yang, Dan;  Anderton, Holly;  Silke, Natasha;  Wachsmuth, Laurens;  Zheng, Lixin;  Moura, Natalia Sampaio;  Beck, David B.;  Gutierrez-Cruz, Gustavo;  Ombrello, Amanda K.;  Pinto-Patarroyo, Gineth P.;  Kueh, Andrew J.;  Herold, Marco J.;  Hall, Cathrine;  Wang, Hongying;  Chae, Jae Jin;  Dmitrieva, Natalia I.;  McKenzie, Mark;  Light, Amanda;  Barham, Beverly K.;  Jones, Anne;  Romeo, Tina M.;  Zhou, Qing;  Aksentijevich, Ivona;  Mullikin, James C.;  Gross, Andrew J.;  Shum, Anthony K.;  Hawkins, Edwin D.;  Masters, Seth L.;  Lenardo, Michael J.;  Boehm, Manfred;  Rosenzweig, Sergio D.;  Pasparakis, Manolis;  Voss, Anne K.;  Gadina, Massimo;  Kastner, Daniel L.;  Silke, John
收藏  |  浏览/下载:70/0  |  提交时间:2020/07/03

RIPK1 is a key regulator of innate immune signalling pathways. To ensure an optimal inflammatory response, RIPK1 is regulated post-translationally by well-characterized ubiquitylation and phosphorylation events, as well as by caspase-8-mediated cleavage1-7. The physiological relevance of this cleavage event remains unclear, although it is thought to inhibit activation of RIPK3 and necroptosis8. Here we show that the heterozygous missense mutations D324N, D324H and D324Y prevent caspase cleavage of RIPK1 in humans and result in an early-onset periodic fever syndrome and severe intermittent lymphadenopathy-a condition we term '  cleavage-resistant RIPK1-induced autoinflammatory syndrome'  . To define the mechanism for this disease, we generated a cleavage-resistant Ripk1(D325A) mutant mouse strain. Whereas Ripk1(-/-) mice died postnatally from systemic inflammation, Ripk1(D325A/D325A) mice died during embryogenesis. Embryonic lethality was completely prevented by the combined loss of Casp8 and Ripk3, but not by loss of Ripk3 or Mlkl alone. Loss of RIPK1 kinase activity also prevented Ripk1(D325A/D325A) embryonic lethality, although the mice died before weaning from multi-organ inflammation in a RIPK3-dependent manner. Consistently, Ripk1(D325A/D325A) and Ripk1(D325A/+) cells were hypersensitive to RIPK3-dependent TNF-induced apoptosis and necroptosis. Heterozygous Ripk1(D325A/+) mice were viable and grossly normal, but were hyper-responsive to inflammatory stimuli in vivo. Our results demonstrate the importance of caspase-mediated RIPK1 cleavage during embryonic development and show that caspase cleavage of RIPK1 not only inhibits necroptosis but also maintains inflammatory homeostasis throughout life.


  
Observation of Bose-Einstein condensates in an Earth-orbiting research lab 期刊论文
NATURE, 2020, 582 (7811) : 103-+
作者:  Yamamoto, Keisuke;  Venida, Anthony;  Yano, Julian;  Biancur, Douglas E.;  Kakiuchi, Miwako;  Gupta, Suprit;  Sohn, Albert S. W.;  Mukhopadhyay, Subhadip;  Lin, Elaine Y.;  Parker, Seth J.;  Banh, Robert S.;  Paulo, Joao A.;  Wen, Kwun Wah;  Debnath, Jayanta;  Kim, Grace E.;  Mancias, Joseph D.;  Fearon, Douglas T.;  Perera, Rushika M.;  Kimmelman, Alec C.
收藏  |  浏览/下载:46/0  |  提交时间:2020/07/03

Quantum mechanics governs the microscopic world, where low mass and momentum reveal a natural wave-particle duality. Magnifying quantum behaviour to macroscopic scales is a major strength of the technique of cooling and trapping atomic gases, in which low momentum is engineered through extremely low temperatures. Advances in this field have achieved such precise control over atomic systems that gravity, often negligible when considering individual atoms, has emerged as a substantial obstacle. In particular, although weaker trapping fields would allow access to lower temperatures(1,2), gravity empties atom traps that are too weak. Additionally, inertial sensors based on cold atoms could reach better sensitivities if the free-fall time of the atoms after release from the trap could be made longer(3). Planetary orbit, specifically the condition of perpetual free-fall, offers to lift cold-atom studies beyond such terrestrial limitations. Here we report production of rubidium Bose-Einstein condensates (BECs) in an Earth-orbiting research laboratory, the Cold Atom Lab. We observe subnanokelvin BECs in weak trapping potentials with free-expansion times extending beyond one second, providing an initial demonstration of the advantages offered by a microgravity environment for cold-atom experiments and verifying the successful operation of this facility. With routine BEC production, continuing operations will support long-term investigations of trap topologies unique to microgravity(4,5), atom-laser sources(6), few-body physics(7,8)and pathfinding techniques for atom-wave interferometry(9-12).


  
Silver-spoon upbringing improves early-life fitness but promotes reproductive ageing in a wild bird 期刊论文
ECOLOGY LETTERS, 2020, 23 (6) : 994-1002
作者:  Spagopoulou, Foteini;  Teplitsky, Celine;  Lind, Martin, I;  Chantepie, Stephane;  Gustafsson, Lars;  Maklakov, Alexei A.
收藏  |  浏览/下载:11/0  |  提交时间:2020/05/13
Ageing  brood size manipulation  condition dependence  disposable soma theory  early-life conditions  senescence  '  silver-spoon'  theory  
A study of ensemble-sensitivity-based initial condition perturbation methods for convection-permitting ensemble forecasts 期刊论文
ATMOSPHERIC RESEARCH, 2020, 234
作者:  Zhang, Xinyan;  Min, Jinzhong;  Wu, Tianjie
收藏  |  浏览/下载:35/0  |  提交时间:2020/07/02
Ensemble-based sensitivity analysis  Initial condition perturbations  Convection-permitting ensemble forecasts  
Vegetation changes attributable to refugees in Africa coincide with agricultural deforestation 期刊论文
ENVIRONMENTAL RESEARCH LETTERS, 2020, 15 (4)
作者:  Maystadt, Jean-Francois;  Mueller, Valerie;  Van den Hoek, Jamon;  van Weezel, Stijn
收藏  |  浏览/下载:21/0  |  提交时间:2020/07/02
refugees  environment  vegetation condition  Africa  
New Analytical Models for Flow Induced by Pumping in a Stream-Aquifer System: A New Robin Boundary Condition Reflecting Joint Effect of Streambed Width and Storage 期刊论文
WATER RESOURCES RESEARCH, 2020, 56 (4)
作者:  Huang, Ching-Sheng;  Wang, Zicheng;  Lin, Ye-Chen;  Yeh, Hund-Der;  Yang, Tao
收藏  |  浏览/下载:20/0  |  提交时间:2020/07/02
stream depletion rate (SDR)  stream-aquifer interaction  Robin boundary condition  lagging theory  streambed storage effect