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DOE公布新的核聚变能源战略 快报文章
地球科学快报,2024年第12期
作者:  刘文浩
Microsoft Word(21Kb)  |  收藏  |  浏览/下载:699/0  |  提交时间:2024/06/25
DOE  Fusion Energy Strategy  
美国核聚变技术仍然面临根本性挑战 快报文章
气候变化快报,2023年第08期
作者:  秦冰雪
Microsoft Word(17Kb)  |  收藏  |  浏览/下载:572/0  |  提交时间:2023/04/20
Nuclear Fusion  Electricity  
英国发布《英国聚变战略》 快报文章
气候变化快报,2021年第20期
作者:  刘燕飞
Microsoft Word(16Kb)  |  收藏  |  浏览/下载:734/0  |  提交时间:2021/10/20
Fusion Energy  Clean Power Solution  Fusion Strategy  
Coupling delay controls synchronized oscillation in the segmentation clock 期刊论文
NATURE, 2020
作者:  Yoshioka-Kobayashi, Kumiko;  Matsumiya, Marina;  Niino, Yusuke;  Isomura, Akihiro;  Kori, Hiroshi;  Miyawaki, Atsushi;  Kageyama, Ryoichiro
收藏  |  浏览/下载:34/0  |  提交时间:2020/07/03

Individual cellular activities fluctuate but are constantly coordinated at the population level via cell-cell coupling. A notable example is the somite segmentation clock, in which the expression of clock genes (such as Hes7) oscillates in synchrony between the cells that comprise the presomitic mesoderm (PSM)(1,2). This synchronization depends on the Notch signalling pathway  inhibiting this pathway desynchronizes oscillations, leading to somite fusion(3-7). However, how Notch signalling regulates the synchronicity of HES7 oscillations is unknown. Here we establish a live-imaging system using a new fluorescent reporter (Achilles), which we fuse with HES7 to monitor synchronous oscillations in HES7 expression in the mouse PSM at a single-cell resolution. Wild-type cells can rapidly correct for phase fluctuations in HES7 oscillations, whereas the absence of the Notch modulator gene lunatic fringe (Lfng) leads to a loss of synchrony between PSM cells. Furthermore, HES7 oscillations are severely dampened in individual cells of Lfng-null PSM. However, when Lfng-null PSM cells were completely dissociated, the amplitude and periodicity of HES7 oscillations were almost normal, which suggests that LFNG is involved mostly in cell-cell coupling. Mixed cultures of control and Lfng-null PSM cells, and an optogenetic Notch signalling reporter assay, revealed that LFNG delays the signal-sending process of intercellular Notch signalling transmission. These results-together with mathematical modelling-raised the possibility that Lfng-null PSM cells shorten the coupling delay, thereby approaching a condition known as the oscillation or amplitude death of coupled oscillators(8). Indeed, a small compound that lengthens the coupling delay partially rescues the amplitude and synchrony of HES7 oscillations in Lfng-null PSM cells. Our study reveals a delay control mechanism of the oscillatory networks involved in somite segmentation, and indicates that intercellular coupling with the correct delay is essential for synchronized oscillation.


Monitoring cells of the mouse presomitic mesoderm using the Achilles reporter fused to HES7 sheds light on the mechanisms that underpin synchronous oscillations in the expression of clock genes between neighbouring cells.


  
Structural basis of DNA targeting by a transposon-encoded CRISPR-Cas system 期刊论文
NATURE, 2020, 577 (7789) : 271-+
作者:  Halpin-Healy, Tyler S.;  Klompe, Sanne E.;  Sternberg, Samuel H.;  Fernandez, Israel S.
收藏  |  浏览/下载:28/0  |  提交时间:2020/07/03

Bacteria use adaptive immune systems encoded by CRISPR and Cas genes to maintain genomic integrity when challenged by pathogens and mobile genetic elements(1-3). Type I CRISPR-Cas systems typically target foreign DNA for degradation via joint action of the ribonucleoprotein complex Cascade and the helicase-nuclease Cas3(4,5), but nuclease-deficient type I systems lacking Cas3 have been repurposed for RNA-guided transposition by bacterial Tn7-like transposons(6,7). How CRISPR- and transposon-associated machineries collaborate during DNA targeting and insertion remains unknown. Here we describe structures of a TniQ-Cascade complex encoded by the Vibrio cholerae Tn6677 transposon using cryo-electron microscopy, revealing the mechanistic basis of this functional coupling. The cryo-electron microscopy maps enabled de novo modelling and refinement of the transposition protein TniQ, which binds to the Cascade complex as a dimer in a head-to-tail configuration, at the interface formed by Cas6 and Cas7 near the 3'  end of the CRISPR RNA (crRNA). The natural Cas8-Cas5 fusion protein binds the 5'  crRNA handle and contacts the TniQ dimer via a flexible insertion domain. A target DNA-bound structure reveals critical interactions necessary for protospacer-adjacent motif recognition and R-loop formation. This work lays the foundation for a structural understanding of how DNA targeting by TniQ-Cascade leads to downstream recruitment of additional transposase proteins, and will guide protein engineering efforts to leverage this system for programmable DNA insertions in genome-engineering applications.


  
Structural transitions in influenza haemagglutinin at membrane fusion pH 期刊论文
NATURE, 2020, 583 (7814) : 150-+
作者:  Wei, Kevin;  Korsunsky, Ilya;  Marshall, Jennifer L.;  Gao, Anqi;  Watts, Gerald F. M.;  Major, Triin;  Croft, Adam P.;  Watts, Jordan;  Blazar, Philip E.;  Lange, Jeffrey K.;  Thornhill, Thomas S.;  Filer, Andrew;  Raza, Karim;  Donlin, Laura T.;  Siebel, Christian W.
收藏  |  浏览/下载:55/0  |  提交时间:2020/07/03

Cryo-electron microscopy studies of the influenza haemagglutinin glycoprotein at the low pH of host endosomes reveals structural intermediates, offering a dynamic view of how the protein mediates membrane fusion.


Infection by enveloped viruses involves fusion of their lipid envelopes with cellular membranes to release the viral genome into cells. For HIV, Ebola, influenza and numerous other viruses, envelope glycoproteins bind the infecting virion to cell-surface receptors and mediate membrane fusion. In the case of influenza, the receptor-binding glycoprotein is the haemagglutinin (HA), and following receptor-mediated uptake of the bound virus by endocytosis(1), it is the HA that mediates fusion of the virus envelope with the membrane of the endosome(2). Each subunit of the trimeric HA consists of two disulfide-linked polypeptides, HA1 and HA2. The larger, virus-membrane-distal, HA1 mediates receptor binding  the smaller, membrane-proximal, HA2 anchors HA in the envelope and contains the fusion peptide, a region that is directly involved in membrane interaction(3). The low pH of endosomes activates fusion by facilitating irreversible conformational changes in the glycoprotein. The structures of the initial HA at neutral pH and the final HA at fusion pH have been investigated by electron microscopy(4,5) and X-ray crystallography(6-8). Here, to further study the process of fusion, we incubate HA for different times at pH 5.0 and directly image structural changes using single-particle cryo-electron microscopy. We describe three distinct, previously undescribed forms of HA, most notably a 150 angstrom-long triple-helical coil of HA2, which may bridge between the viral and endosomal membranes. Comparison of these structures reveals concerted conformational rearrangements through which the HA mediates membrane fusion.


  
Hummingbird-sized dinosaur from the Cretaceous period of Myanmar 期刊论文
NATURE, 2020, 579 (7798) : 245-+
作者:  McBrien, Julia Bergild;  Mavigner, Maud;  Franchitti, Lavinia;  Smith, S. Abigail;  White, Erick;  Tharp, Gregory K.;  Walum, Hasse;  Busman-Sahay, Kathleen;  Aguilera-Sandoval, Christian R.;  Thayer, William O.;  Spagnuolo, Rae Ann;  Kovarova, Martina;  Wahl, Angela;  Cervasi, Barbara;  Margolis, David M.
收藏  |  浏览/下载:58/0  |  提交时间:2020/05/13

Skeletal inclusions in approximately 99-million-year-old amber from northern Myanmar provide unprecedented insights into the soft tissue and skeletal anatomy of minute fauna, which are not typically preserved in other depositional environments(1-3). Among a diversity of vertebrates, seven specimens that preserve the skeletal remains of enantiornithine birds have previously been described(1,4-8), all of which (including at least one seemingly mature specimen) are smaller than specimens recovered from lithic materials. Here we describe an exceptionally well-preserved and diminutive bird-like skull that documents a new species, which we name Oculudentavis khaungraae gen. et sp. nov. The find appears to represent the smallest known dinosaur of the Mesozoic era, rivalling the bee hummingbird (Mellisuga helenae)-the smallest living bird-in size. The O. khaungraae specimen preserves features that hint at miniaturization constraints, including a unique pattern of cranial fusion and an autapomorphic ocular morphology(9) that resembles the eyes of lizards. The conically arranged scleral ossicles define a small pupil, indicative of diurnal activity. Miniaturization most commonly arises in isolated environments, and the diminutive size of Oculudentavis is therefore consistent with previous suggestions that this amber formed on an island within the Trans-Tethyan arc(10). The size and morphology of this species suggest a previously unknown bauplan, and a previously undetected ecology. This discovery highlights the potential of amber deposits to reveal the lowest limits of vertebrate body size.


  
Multiscale Data Fusion for Surface Soil Moisture Estimation: A Spatial Hierarchical Approach 期刊论文
WATER RESOURCES RESEARCH, 2019, 55 (12) : 10443-10465
作者:  Kathuria, Dhruva;  Mohanty, Binayak P.;  Katzfuss, Matthias
收藏  |  浏览/下载:32/0  |  提交时间:2020/02/16
soil moisture remote sensing  scaling  multi-instrument data fusion  spatial hierarchical model  uncertainty  physical controls  
Regional Biases in MODIS Marine Liquid Water Cloud Drop Effective Radius Deduced Through Fusion With MISR 期刊论文
JOURNAL OF GEOPHYSICAL RESEARCH-ATMOSPHERES, 2019
作者:  Fu, Dongwei;  Di Girolamo, Larry;  Liang, Lusheng;  Zhao, Guangyu
收藏  |  浏览/下载:35/0  |  提交时间:2020/02/17
MISR  MODIS  effective radius  liquid clouds  bias-correction  data fusion  
Impact of Rescaling Approaches in Simple Fusion of Soil Moisture Products 期刊论文
WATER RESOURCES RESEARCH, 2019, 55 (9) : 7804-7825
作者:  Afshar, M. H.;  Yilmaz, M. T.;  Crow, W. T.
收藏  |  浏览/下载:19/0  |  提交时间:2019/11/27
data fusion  rescaling  simple merging  soil moisture