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Coupling delay controls synchronized oscillation in the segmentation clock 期刊论文
NATURE, 2020
作者:  Yoshioka-Kobayashi, Kumiko;  Matsumiya, Marina;  Niino, Yusuke;  Isomura, Akihiro;  Kori, Hiroshi;  Miyawaki, Atsushi;  Kageyama, Ryoichiro
收藏  |  浏览/下载:16/0  |  提交时间:2020/07/03

Individual cellular activities fluctuate but are constantly coordinated at the population level via cell-cell coupling. A notable example is the somite segmentation clock, in which the expression of clock genes (such as Hes7) oscillates in synchrony between the cells that comprise the presomitic mesoderm (PSM)(1,2). This synchronization depends on the Notch signalling pathway  inhibiting this pathway desynchronizes oscillations, leading to somite fusion(3-7). However, how Notch signalling regulates the synchronicity of HES7 oscillations is unknown. Here we establish a live-imaging system using a new fluorescent reporter (Achilles), which we fuse with HES7 to monitor synchronous oscillations in HES7 expression in the mouse PSM at a single-cell resolution. Wild-type cells can rapidly correct for phase fluctuations in HES7 oscillations, whereas the absence of the Notch modulator gene lunatic fringe (Lfng) leads to a loss of synchrony between PSM cells. Furthermore, HES7 oscillations are severely dampened in individual cells of Lfng-null PSM. However, when Lfng-null PSM cells were completely dissociated, the amplitude and periodicity of HES7 oscillations were almost normal, which suggests that LFNG is involved mostly in cell-cell coupling. Mixed cultures of control and Lfng-null PSM cells, and an optogenetic Notch signalling reporter assay, revealed that LFNG delays the signal-sending process of intercellular Notch signalling transmission. These results-together with mathematical modelling-raised the possibility that Lfng-null PSM cells shorten the coupling delay, thereby approaching a condition known as the oscillation or amplitude death of coupled oscillators(8). Indeed, a small compound that lengthens the coupling delay partially rescues the amplitude and synchrony of HES7 oscillations in Lfng-null PSM cells. Our study reveals a delay control mechanism of the oscillatory networks involved in somite segmentation, and indicates that intercellular coupling with the correct delay is essential for synchronized oscillation.


Monitoring cells of the mouse presomitic mesoderm using the Achilles reporter fused to HES7 sheds light on the mechanisms that underpin synchronous oscillations in the expression of clock genes between neighbouring cells.


  
Demographic costs and benefits of natural regeneration during tropical forest restoration 期刊论文
ECOLOGY LETTERS, 2019, 22 (1) : 34-44
作者:  Caughlin, T. Trevor;  de la Pena-Domene, Marines;  Martinez-Garza, Cristina
收藏  |  浏览/下载:14/0  |  提交时间:2019/04/09
Demographic model  early succession  full life-cycle analysis  individual-based model  life-history categories  natural regeneration  population-level  reforestation  successional age  tropical forest restoration  
Extremely low genetic diversity across mangrove taxa reflects past sea level changes and hints at poor future responses 期刊论文
GLOBAL CHANGE BIOLOGY, 2018, 24 (4) : 1741-1748
作者:  Guo, Zixiao;  Li, Xinnian;  He, Ziwen;  Yang, Yuchen;  Wang, Wenqing;  Zhong, Cairong;  Greenberg, Anthony J.;  Wu, Chung-I;  Duke, Norman C.;  Shi, Suhua
收藏  |  浏览/下载:26/0  |  提交时间:2019/04/09
dieback  effective population size  genetic diversity  human disturbance  mangroves  sea level rise  
Peri-urban land use pattern and its relation to land use planning in Ghana, West Africa 期刊论文
LANDSCAPE AND URBAN PLANNING, 2017, 165
作者:  Kleemann, Janina;  Inkoorn, Justice Nana;  Thiel, Michael;  Shankar, Sangeetha;  Lautenbach, Sven;  Fuerst, Christine
收藏  |  浏览/下载:10/0  |  提交时间:2019/04/09
Population growth  Urban development  Urban sprawl  Land tenure  Land use change  Drivers  Confidence level analysis  Remote sensing  Interviews  
High-resolution tide projections reveal extinction threshold in response to sea-level rise 期刊论文
GLOBAL CHANGE BIOLOGY, 2017, 23 (5)
作者:  Field, Christopher R.;  Bayard, Trina S.;  Gjerdrum, Carina;  Hill, Jason M.;  Meiman, Susan;  Elphick, Chris S.
收藏  |  浏览/下载:29/0  |  提交时间:2019/04/09
extinction threshold  population viability  saltmarsh sparrow  sea-level rise  tidal marsh  tide projection  
The human core of the shared socioeconomic pathways: Population scenarios by age, sex and level of education for all countries to 2100 期刊论文
GLOBAL ENVIRONMENTAL CHANGE-HUMAN AND POLICY DIMENSIONS, 2017, 42
作者:  Samir, K. C.;  Lutz, Wolfgang
收藏  |  浏览/下载:23/0  |  提交时间:2019/04/09
World population  Education  Age structure  Scenarios  Country level  Shared socioeconomic pathways